VHR/DUSP3 Antibody (5B7)

Western Blot: VHR/DUSP3 Antibody (5B7) [H00001845-M01] - Analysis of DUSP3 expression in transfected 293T cell line by DUSP3 monoclonal antibody (M01), clone 5B7.Lane 1: DUSP3 transfected lysate(20.478 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: VHR/DUSP3 Antibody (5B7) [H00001845-M01] - Analysis of monoclonal antibody to DUSP3 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: VHR/DUSP3 Antibody (5B7) [H00001845-M01] - Detection limit for recombinant GST tagged DUSP3 is approximately 1ng/ml as a capture antibody.

Product Discontinued

VHR/DUSP3 Antibody (5B7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DUSP3 (AAH02682, 1 a.a. ~ 185 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEGKLKP
• Specificity: DUSP3 - dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) (5B7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DUSP3
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VHR/DUSP3 Antibody (5B7)

• dual specificity phosphatase 3
• dual specificity protein phosphatase 3
• Dual specificity protein phosphatase VHR
• DUSP3
• EC 3.1.3.16
• EC 3.1.3.48
• Vaccinia H1-related phosphatase
• vaccinia virus phosphatase VH1-related
• VHR
• VHRserine/threonine specific protein phosphatase

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP3
• OMIM:
  • 600183(Human)