Vanilloid R1/TRPV1 Antibody

Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-1617] - CGRP and TRPV1 are co-expressed in most cultured trigeminal ganglion neurons. (A and C) CGRP expression in d2 trigeminal ganglion neurons plated on poly-d-lysine-coated coverslips. (B and D) The same culture shown in panel A or C costained for TRPV1 expression. Magnification bar = 200m (A and B) or 40m (C and D)

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NB100-1617] - TRPV1 Antibody [NB100-1617] - Dorsal Root Ganglion

Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-1617] - TRPV1 staining in rat trigeminal ganglion. Image courtesy of Dr. Norman Capra, University of Maryland

• Reactivity: Hu, Rt, Pm
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Vanilloid R1/TRPV1 Antibody Summary

• Immunogen: Synthetic peptide: RASLDSEESESPPQENSC, corresponding to amino-terminus of Rat VR1, amino acids 4-21.
• Localization: Plasma membrane
• Specificity: Vanilloid Receptor 1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPV1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
• Application Notes: Immunohistochemistry and Immunocytochemistry 1:1000. Dilution listed only as a recommendation. Optimal dilution should be determined by investigator. IHC-Frozen reactivity reported in scientific literature (PMID: 23573230), Immunoprecipitation reactivity reported in scientific literature (PMID: 25801060)
• Positive Control:

  Vanilloid R1/TRPV1 Lysate (NBP2-65958)

• Control Peptide:

  Vanilloid R1/TRPV1 Protein (NB100-1617PEP)

Reactivity Notes

Human and Primate reactivity reported in scientific literature (PMID: 25713995). Mouse reactivity is reported in scientific literature (PMID: 25801060), but this antibody may not be suitable for detection of the mouse protein in WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Vanilloid R1/TRPV1 Antibody

• Capsaicin Receptor
• Osm-9-like TRP channel 1
• OTRPC1
• transient receptor potential cation channel subfamily V member 1
• transient receptor potential cation channel, subfamily V, member 1
• transient receptor potential vanilloid 1a
• transient receptor potential vanilloid 1b
• TRPV1
• Vanilloid R1
• Vanilloid receptor 1
• vanilloid receptor subtype 1
• VanilloidR1
• VR1
• VR1DKFZp434K0220

Background

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vanilloid R1/TRPV1 Antibody (NB100-1617)(15)

Publications using NB100-1617

• Huang WX, Yu F, Sanchez RM et al. TRPV1 promotes repetitive febrile seizures by pro-inflammatory cytokines in immature brain. Brain Behav. Immun. 2015 Mar 20 [PMID: 25801060] (IP, Mouse)
• Sappington RM, Sidorova T, Ward NJ et al. Activation of Transient Receptor Potential Vanilloid-1 (TRPV1) Influences How Retinal Ganglion Cell Neurons Respond to Pressure-Related Stress Channels (Austin). 2015 Feb 25 [PMID: 25713995] (WB, Human, Rat, Primate)
• Zhao Z, Fan H, Higgins T et al. Fufang Kushen injection inhibits sarcoma growth and tumor-induced hyperalgesia via TRPV1 signaling pathways. Cancer Lett. 2014 Sep 19 [PMID: 25242356]
• Starowicz K, Makuch W, Korostynski M et al. Full Inhibition of Spinal FAAH Leads to TRPV1-Mediated Analgesic Effects in Neuropathic Rats and Possible Lipoxygenase-Mediated Remodeling of Anandamide Metabolism PLoS One 2013 [PMID: 23573230] (IHC-Fr, ICC/IF, Rat)
• Sappington RM, Sidorova T, Long DJ et al. TRPV1: Contribution to Retinal Ganglion Cell Apoptosis Increased Intracellular Ca2+ with Exposure to Hydrostatic Pressure. Invest Ophthalmol Vis Sci;50(2):717-28. 2009 Feb. [PMID: 18952924]
• Sappington RM Calkins DJ. Contribution of TRPV1 to Microglia-Derived IL-6 NF{kappa}B Translocation with Elevated Hydrostatic Pressure. Invest Ophthalmol Vis Sci;49(7):3004-3017. 2008 [PMID: 18362111]
• Stein, A et al. Phosphoinositide 3-Kinase Binds to TRPV1 Mediates NGF-stimulated TRPV1 Trafficking to the Plasma Membrane. Published online doi:10.1085/jgp.200609576. 2006 Oct 30. [PMID: 17074976]
• Sharif R et al. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nature Neuroscience; (1):93-8. 2006 [PMID: 16327782]
• Liapi A et al. Extensive co-localization heteromultimer formation of the vanilloid receptor-like protein TRPV2 the capsaicin receptor TRPV1 in the adult rat cerebral cortex. Eur J Neurosci; 22(4):825. 2005 [PMID: 16115206]
• Aoki Y et al. Expression Co-Expression of VR1, CGRP, IB4-Binding Glycoprotein in Dorsal Root Ganglion Neurons in Rats: Differences Between the Disc Afferents the Cutaneous Afferents. Spine; 30(13):1496-1500. 2005 [PMID: 15990662]
• 9 Zimov S Yazulla S. Localization of vanilloid receptor 1 (TRPV1/VR1)-like immunoreactivity in goldfish zebrafish retinas: Restriction to photoreceptor synaptic ribbons. J Neurocytol; 33:441. 2004 [PMID: 15520529]
• Zahner MR, Li DP, Chen SR, Pan HL. Cardiac vanilloid receptor 1-expressing afferent nerves their role in the cardiogenic sympathetic reflex in rats. J Physiol; 551(2):515-523. 2003 [PMID: 12829722]
• Nakatsuka T et al. Distinct Roles of P2X Receptors in Modulating Glutamate Release at Different Primary Sensory Synapses in Rat Spinal Cord. J Neurophys; 89: 3243-3252. 2003 [PMID: 12783958]
• Labrakakis C et al. Localization function of ATP GABAA receptors expressed by nociceptors other postnatal sensory neurons in rat. J Physiol; 549(1):131-142. 2003 [PMID: 12665615]
• Rosenbaum T, Awaya M, Gordon SE. Subunit modification association in VR1 ion channels. BMC Neurosci, 3:4. 2002 [PMID: 11914134]

Bioinformatics

• Gene Symbol: TRPV1
• Entrez:
  • 7442(Human)
  • 193034(Mouse)
  • 83810(Rat)
• Uniprot:
  • O35433()