VAMP-1/VAMP-2 Antibody (SP10)

Images

 
Western Blot: VAMP-2 Antibody (SP10) [NBP1-19332] - Analysis of mouse brain tissue extract.
Immunohistochemistry-Paraffin: VAMP-2 Antibody (SP10) [NBP1-19332] - Analysis of mouse brain tissue extract
Immunohistochemistry: VAMP-2 Antibody (SP10) [NBP1-19332] - Analysis of Xenopus embryonic brain, stained with VAMP2, mAb (SP10).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ha, XpSpecies Glossary
Applications WB, IHC, IHC-P
Clone
SP10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

VAMP-1/VAMP-2 Antibody (SP10) Summary

Immunogen
Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies.
Marker
Synaptic Vesicles Marker
Specificity
VAMP2 (SP10)
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
VAMP1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:4000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.1mM PMSF and 50% Glycerol
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for VAMP-1/VAMP-2 Antibody (SP10)

  • VAMP-1/VAMP-2

Background

VAMP2 (Synaptobrevin 2) is a small integral membrane protein found specifically in synaptic vesicles in neurons. Synaptobrevins/VAMPs, syntaxins, and the 25 kD synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP2 is one of a family of integral membrane proteins of 11-13 kDa with the N terminal region exposed to the cytoplasm and a C terminal transmembrane domain. Two isoforms identified in the mammalian CNS Synaptobrevin 1/VAMP 1 and Synaptobrevin 2/VAMP 2 differ in their distribution within different brain regions. Synaptobrevin/VAMP is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2/VAMP 2 are present in many other tissues where the protein resides on specialized microvesicles. In non-neuronal cells, the third isoform, Cellubrevin/VAMP 3, is present where it is localized to an endosomal membrane pool. Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C terminal portion of the molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-595
Species: Mu, Rt, Bv
Applications: WB, Flow, IHC, IHC-P, IP, IF
NBP2-00795
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-510
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF
NBP1-91499
Species: Mu, Bv
Applications: WB, ICC/IF, IHC
NB300-653
Species: Hu, Mu, Rt, Ch, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-87035
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87374
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IF
AF7237
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP1-83162
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-46627
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB120-15855
Species: Hu, Mu, Rt, Bv
Applications: WB
AF5085
Species: Hu, Mu, Rt
Applications: WB, ICC
MAB6117
Species: Hu
Applications: WB, IHC
NBP2-46628
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
NBP2-33414
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF5675
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP1-86101
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for VAMP-1/VAMP-2 Antibody (NBP1-19332) (0)

There are no publications for VAMP-1/VAMP-2 Antibody (NBP1-19332).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VAMP-1/VAMP-2 Antibody (NBP1-19332) (0)

There are no reviews for VAMP-1/VAMP-2 Antibody (NBP1-19332). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VAMP-1/VAMP-2 Antibody (NBP1-19332) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional VAMP-1/VAMP-2 Products

VAMP-1/VAMP-2 NBP1-19332

Bioinformatics Tool for VAMP-1/VAMP-2 Antibody (NBP1-19332)

Discover related pathways, diseases and genes to VAMP-1/VAMP-2 Antibody (NBP1-19332). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VAMP-1/VAMP-2 Antibody (NBP1-19332)

Discover more about diseases related to VAMP-1/VAMP-2 Antibody (NBP1-19332).
 

Pathways for VAMP-1/VAMP-2 Antibody (NBP1-19332)

View related products by pathway.

PTMs for VAMP-1/VAMP-2 Antibody (NBP1-19332)

Learn more about PTMs related to VAMP-1/VAMP-2 Antibody (NBP1-19332).

Blogs on VAMP-1/VAMP-2

There are no specific blogs for VAMP-1/VAMP-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VAMP-1/VAMP-2 Antibody (SP10) and receive a gift card or discount.

Bioinformatics

Gene Symbol VAMP1
Entrez