VAMP-1 Antibody (JG40-19)

Western Blot: VAMP-1 Antibody (JG40-19) [NBP2-76877] - Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution.Positive control:Lane 1: Mouse cerebellumLane 2: Rat brain

Immunocytochemistry: VAMP-1 Antibody (JG40-19) [NBP2-76877] - ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: VAMP-1 Antibody (JG40-19) [NBP2-76877] - Analysis of mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.

Immunocytochemistry: VAMP-1 Antibody (JG40-19) [NBP2-76877] - ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry: VAMP-1 Antibody (JG40-19) [NBP2-76877] - ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: VAMP-1 Antibody (JG40-19) [NBP2-76877] - Analysis of human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.

Product Discontinued

VAMP-1 Antibody (JG40-19) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant full length protein of human VAMP-1. (SwissProt: P23763 Human; SwissProt: Q62442 Mouse; SwissProt: Q63666 Rat)
• Localization: Mitochondrion. Synapse. Synaptosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VAMP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for VAMP-1 Antibody (JG40-19)

• DKFZp686H12131
• SYB1
• SYB1vesicle-associated membrane protein 1
• synaptobrevin 1
• Synaptobrevin-1
• VAMP1
• VAMP-1
• VAMP-1synaptobrevin-1
• vesicle-associated membrane protein 1 (synaptobrevin 1)

Background

The Vesicle Associated Membrane Proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer®s disease, VAMP levels of all isoforms appear to be significantly lowered. It has been shown that the extreme carboxy-terminus of VAMP-1 is involved in subcellular vesicle targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAMP1