V1b Vasopressin R/AVPR1B Antibody

Western Blot: V1b Vasopressin R/AVPR1B Antibody [NB100-96917] - (0.5ug/ml) Analysis of Human Amygdala lysate (35ug protein in RIPA buffer) in lane A and (0.05ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer) in lane B . Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: V1b Vasopressin R/AVPR1B Antibody [NB100-96917] - (5ug/ml) staining Human Anterior Pituitary Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Discontinued

V1b Vasopressin R/AVPR1B Antibody Summary

• Immunogen: Peptide with sequence C-PRDLELADGEGTAE corresponding to C-Terminus according to NP_000698.1.
• Epitope: PRDLELADGEGTAE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AVPR1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.05 - 0.2 ug/ml
• Application Notes: WB: Approx. 170 kDa band observed in human brain (substantia nigra) lysates (calculated MW of 47.0 kDa band according to NP_000698.1). The observed molecular weight corresponds to findings with EB08786, a product of different design reacting to the mouse protein. IHC-P: Human Pituitary shows bright cytoplasm staining of a few active secretory cells in the anterior pituitary.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for V1b Vasopressin R/AVPR1B Antibody

• Antidiuretic hormone receptor 1b
• arginine vasopressin receptor 1B
• AVPR V1b
• AVPR V3
• AVPR1B
• AVPR3
• AVPR3arginine vasopressin receptor 3
• pituitary vasopressin receptor 3
• V1b Vasopressin R
• V1b VasopressinR
• V1bR
• vasopressin V1b receptor
• Vasopressin V3 receptor
• VPR3

Background

The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1A, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor is primarily located in the anterior pituitary, where it stimulates ACTH release. It is expressed at high levels in ACTH-secreting pituitary adenomas as well as in bronchial carcinoids responsible for the ectopic ACTH syndrome. A spliced antisense transcript of this gene has been reported but its function is not known. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for V1b Vasopressin R/AVPR1B Antibody (NB100-96917)(1)

Publication using NB100-96917

• Dempster EL, Burcescu I, Wigg K et al. Evidence of an association between the vasopressin V1b receptor gene (AVPR1B) and childhood-onset mood disorders. Arch Gen Psychiatry 2007-10-01 [PMID: 17909131]

Bioinformatics

• Gene Symbol: AVPR1B
• Entrez:
  • 553(Human)
• Uniprot:
  • NP_000698.1(Human)