USP9x Knockout HeLa Cell Lysate

Product Discontinued

USP9x Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-1 bp deletion in exon9 and 1 bp insertion in exon9
• Gene: USP9X

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for USP9x Knockout HeLa Cell Lysate

• Deubiquitinating enzyme FAF-X
• DFFRX
• DFFRXubiquitin specific protease 9, X-linked (fat facets-like, Drosophila)
• Drosophila fat facets related, X-linked
• EC 3.1.2.15
• EC 3.4.19.12
• FAF
• FAM
• Fat facets in mammals
• fat facets protein related, X-linked
• Fat facets protein-related, X-linked
• hFAM
• probable ubiquitin carboxyl-terminal hydrolase FAF-X
• ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
• ubiquitin specific peptidase 9, X-linked
• ubiquitin thioesterase FAF-X
• Ubiquitin thiolesterase FAF-X
• ubiquitin-specific processing protease FAF-X
• Ubiquitin-specific protease 9, X chromosome
• Ubiquitin-specific-processing protease FAF-X
• USP9
• USP9x
• X chromosome (fat facets-like Drosophila)

Background

This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: USP9X