USP3 Antibody (1H2) - Azide and BSA Free

Western Blot: USP3 Antibody (1H2) [H00009960-M01] - Analysis of USP3 expression in transfected 293T cell line by USP3 monoclonal antibody (M01), clone 1H2.Lane 1: USP3 transfected lysate(58.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: USP3 Antibody (1H2) [H00009960-M01] - Analysis of monoclonal antibody to USP3 on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: USP3 Antibody (1H2) [H00009960-M01] - Detection limit for recombinant GST tagged USP3 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

USP3 Antibody (1H2) - Azide and BSA Free Summary

• Immunogen: USP3 (NP_006528, 421 a.a. ~ 520 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNKVDTYVEFPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL
• Specificity: USP3 (1H2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP3 Antibody (1H2) - Azide and BSA Free

• Deubiquitinating enzyme 3
• EC 3.1.2.15
• EC 3.4.19.12
• MGC129878
• MGC129879
• SIH003
• ubiquitin carboxyl-terminal hydrolase 3
• ubiquitin specific peptidase 3
• ubiquitin specific protease 3
• ubiquitin thioesterase 3
• Ubiquitin thiolesterase 3
• Ubiquitin-specific-processing protease 3
• UBP
• USP3

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B. Alternate names for USP3 include ubiquitin carboxyl-terminal hydrolase 3, ubiquitin thioesterase 3, ubiquitin-specific-processing protease 3, and deubiquitinating enzyme 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP3 Antibody (H00009960-M01)(1)

Publication using H00009960-M01

• Mosbech A, Lukas C, Bekker-Jensen S, Mailand N. The deubiquitylating enzyme USP44 counteracts the DNA double-strand break response mediated by the RNF8 and RNF168 ubiquitin ligases. J Biol Chem. 2013-06-07 [PMID: 23615962] (Human)

Bioinformatics

• Gene Symbol: USP3
• Entrez:
  • 9960(Human)
• OMIM:
  • 604728(Human)
• Uniprot:
  • NP_006528(Human)