Use1/UBE2Z Antibody [Unconjugated]

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Western blot shows lysates of A549 human lung carcinoma cell line and PANC‑1 human pancreatic carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Use1/UBE2Z Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Use1/UBE2Z Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Use1/UBE2Z
Met1-Val354
Accession # Q9H832
Specificity
Detects human USE1/UBE2Z in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
UBE2Z
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Use1/UBE2Z Antibody [Unconjugated]

  • MDS032
  • p31
  • protein p31
  • putative MAPK activating protein PM26
  • Putative MAPK-activating protein PM26
  • Q-SNARE
  • SLT1
  • SNARE-like tail-anchored protein 1 homolog
  • UBE2Z
  • unconventional SNARE in the ER 1 homolog (S. cerevisiae)
  • Use1
  • USE1L
  • USE1-like protein
  • vesicle transport protein USE1

Background

Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis.  Use1 has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin.  It functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation.  Expression is enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1 also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma.  Full-length human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse and rat Use1.  A potential smaller isoform lacks aa 1-108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBE2Z
Uniprot