UQCRC2 Antibody

Western Blot: UQCRC2 Antibody [NBP2-76971] - Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.

Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP2-76971] - ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry: UQCRC2 Antibody [NBP2-76971] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.

Flow Cytometry: UQCRC2 Antibody [NBP2-76971] - Flow cytometric analysis of HepG2 cells with UQCRC2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP2-76971] - ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP2-76971] - ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry: UQCRC2 Antibody [NBP2-76971] - Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.

Product Discontinued

UQCRC2 Antibody Summary

• Immunogen: Recombinant protein within human UQCRC2 aa 90-370. (SwissProt: P22695 Human; SwissProt: Q9DB77 Mouse; SwissProt: P32551 Rat)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UQCRC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-2000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UQCRC2 Antibody

• Complex III subunit 2
• Core protein II
• cytochrome b-c1 complex subunit 2, mitochondrial
• QCR2
• ubiquinol-cytochrome c reductase core protein II
• Ubiquinol-cytochrome-c reductase complex core protein 2
• UQCR2

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex),which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UQCRC2