UGT1A6 Overexpression Lysate (Native)

Images

 
Western Blot: UGT1A6 Overexpression Lysate (Adult Normal) [NBL1-17598] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UGT1A6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

UGT1A6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215957 and based on accession number NM_001072. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens UDP glucuronosyltransferase 1 family, polypeptide A6 (UGT1A6), transcript variant 1, mRNA.
Gene
UGT1A6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
57.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for UGT1A6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UGT1A6 Overexpression Lysate (Native)

  • GNT1UDPGT 1-6
  • HLUGP
  • HLUGP1
  • MGC29860
  • Phenol-metabolizing UDP-glucuronosyltransferase
  • UDP glucuronosyltransferase 1 family, polypeptide A6
  • UDP glycosyltransferase 1 family, polypeptide A6
  • UDP-glucuronosyltransferase 1 family polypeptide A6s
  • UDP-glucuronosyltransferase 1-6
  • UDP-glucuronosyltransferase 1A6
  • UDP-glucuronosyltransferase 1-F
  • UDPGT
  • UGT1
  • UGT1*6
  • UGT1.6
  • UGT1-06
  • UGT1A6S
  • UGT-1F
  • UGT1FEC 2.4.1.17

Background

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenolic and planar compounds. Alternative splicing in the unique 5' end of this gene results in two transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Its first exon contains both the 5' UTR and the 5' coding sequence, as is typical of the UGT1A genes. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for UGT1A6 Lysate (NBL1-17598) (0)

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Bioinformatics

Gene Symbol UGT1A6