Recombinant Human Ufc1 Protein

Product Discontinued

Recombinant Human Ufc1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-167 of Human Ufc1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UFC1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Ufc1 Protein

• HSPC155
• ubiquitin-fold modifier conjugating enzyme 1
• ubiquitin-fold modifier-conjugating enzyme 1
• Ufc1
• Ufm1-conjugating enzyme 1

Background

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. UFM1 is activated by a novel E1-like enzyme, UBA5, by forming a high-energy thioester bond. Activated UFM1 is then transferred to its cognate E2-like enzyme, UFC1, in a similar thioester linkage. Recombinant human UFC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UFC1