Recombinant Mouse UCH-L3 His Protein

SDS-Page: UCH-L3 Recombinant Protein [NBP2-52385] - 15% SDS Page (3 ug)

• Reactivity: Mu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Mouse UCH-L3 His Protein Summary

• Description: A bioactive recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-230 of Mouse UCH-L3
  
  

  Source: Baculovirus

  Amino Acid Sequence: MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH

• Details of Functionality: Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: UCHL3
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 27.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mouse UCH-L3 His Protein

• EC 3.4.19.12
• ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L3
• Ubiquitin Thioesterase L3
• ubiquitin thiolesterase
• UCHL3
• UCH-L3

Background

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UCHL3