Recombinant Human UCH-L3 Protein

Product Discontinued

Recombinant Human UCH-L3 Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-230 of Human UCH-L3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

• Details of Functionality: Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UCHL3
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 28.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human UCH-L3 Protein

• EC 3.4.19.12
• ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L3
• Ubiquitin Thioesterase L3
• ubiquitin thiolesterase
• UCHL3
• UCH-L3

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1. Recombinant human UCHL3 protprot, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UCHL3