UCH-L3 Antibody

Immunohistochemistry-Paraffin: UCH-L3 Antibody [NB110-40472] - Analysis of human skeletal muscle.

Product Discontinued

UCH-L3 Antibody Summary

• Immunogen: Synthetic 18 amino acid peptide from internal region of human UCHL3.
• Epitope: internal domain of human
• Specificity: Human UCHL3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Porcine (94%), Bovine (94%), Orangutan (94%), Rabbit (94%), Opossum (100%), Equine (94%), Primate (94%), Turkey (100%), Monkey (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UCHL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Zebrafish (89%), Xenopus (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UCH-L3 Antibody

• EC 3.4.19.12
• ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L3
• Ubiquitin Thioesterase L3
• ubiquitin thiolesterase
• UCHL3
• UCH-L3

Background

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Four distinct UCH activities from bovine thymus have been identified. All 4 are thiol proteases and have high-affinity binding sites for ubiquitin. UCHL3 protein is 54% identical to that of UCHL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCHL3
• Entrez:
  • 7347(Human)