Ubiquitin-activating Enzyme/UBE1 Antibody - BSA Free

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-20779] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ubiquitin-activating Enzyme/UBE1 antibody [N1N3] (NBP2-20779) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Rb
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ubiquitin-activating Enzyme/UBE1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ubiquitin-activating Enzyme/UBE1 Antibody - BSA Free (NBP2-20779) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ubiquitin-activating Enzyme/UBE1. The exact sequence is proprietary.
• Predicted Species: Mouse (91%), Rat (93%), Bovine (94%), Rabbit (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:5000-1:20000
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Ubiquitin-activating Enzyme/UBE1 Antibody - BSA Free

• A1S9T and BN75 temperature sensitivity complementing
• A1S9T
• A1ST
• AMCX1
• GXP1
• MGC4781
• POC20 centriolar protein homolog
• POC20
• Protein A1S9
• SMAX2
• UBA1
• UBA1, ubiquitin-activating enzyme E1 homolog A
• UBA1A
• UBE1
• UBE1A1S9
• UBE1X
• ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
• Ubiquitin-activating enzyme E1
• Ubiquitinactivating Enzyme
• Ubiquitin-activating Enzyme
• ubiquitin-like modifier activating enzyme 1
• ubiquitin-like modifier-activating enzyme 1

Background

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBA1
• Uniprot:
  • P22314(Human)