UBE2T Antibody

Western Blot: UBE2T Antibody [NB100-1150] - Analysis of UBE2T in K562 lysate (35ug protein in RIPA buffer) using NB100-1150 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UBE2T Antibody Summary

• Description: Novus Biologicals Goat UBE2T Antibody (NB100-1150) is a polyclonal antibody validated for use in WB and ELISA. Anti-UBE2T Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QLVGIEKKFHPDV corresponding to C-Terminus according to NP_054895.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBE2T
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in lysates of K562, HEK293 and A549 (calculated MW of 22.5 kDa band according to NP_054895.1).
• Control:

  UBE2T Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBE2T Antibody

• Cell proliferation-inducing gene 50 protein
• EC 6.3.2.19
• HSPC150 protein similar to ubiquitin-conjugating enzyme
• HSPC150
• PIG50
• UBE2T
• Ubiquitin carrier protein T
• ubiquitin conjugating enzyme
• ubiquitin-conjugating enzyme E2 T
• ubiquitin-conjugating enzyme E2T (putative)
• Ubiquitin-protein ligase T

Background

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2T Antibody (NB100-1150)(1)

Publication using NB100-1150

• Machida YJ, Machida Y, Chen Y et al. UBE2T is the E2 in the Fanconi anemia pathway and undergoes negativeautoregulation. Mol Cell 2006-08-01 [PMID: 16916645]

Bioinformatics

• Gene Symbol: UBE2T
• Entrez:
  • 29089(Human)
• Uniprot:
  • NP_054895.(Human)