UBE2R2 Antibody (5E7) - Azide and BSA Free

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01] - UBE2R2 monoclonal antibody (M01), clone 5E7 Analysis of UBE2R2 expression in Jurkat.

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01] - Analysis of UBE2R2 expression in transfected 293T cell line by UBE2R2 monoclonal antibody (M01), clone 5E7.Lane 1: UBE2R2 transfected lysate (Predicted MW: 27.2 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: UBE2R2 Antibody (5E7) [H00054926-M01] - Analysis of UBE2R2 transfected lysate using anti-UBE2R2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2R2 monoclonal antibody.

Sandwich ELISA: UBE2R2 Antibody (5E7) [H00054926-M01] - Detection limit for recombinant GST tagged UBE2R2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 5E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UBE2R2 Antibody (5E7) - Azide and BSA Free Summary

• Immunogen: UBE2R2 (AAH47584, 1 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES
• Specificity: UBE2R2 - ubiquitin-conjugating enzyme E2R 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2R2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UBE2R2 Antibody (5E7) - Azide and BSA Free

• CDC34B
• CDC34BEC 6.3.2.19
• E2-CDC34B
• FLJ20419
• MGC10481
• UBC3B
• UBC3BUbiquitin-protein ligase R2
• UBE2R2
• Ubiquitin carrier protein R2
• ubiquitin-conjugating enzyme E2 R2
• Ubiquitin-conjugating enzyme E2-CDC34B
• ubiquitin-conjugating enzyme E2R 2
• ubiquitin-conjugating enzyme UBC3B

Background

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2R2
• Entrez:
  • 54926(Human)
• Uniprot:
  • AAH47584(Human)