UBE2K/E2-25K Antibody - BSA Free

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: UBE2K/E2-25K Antibody [NBP2-16831] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBE2K/E2-25K Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UBE2K/E2-25K Antibody - BSA Free (NBP2-16831) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2K/E2-25K. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Xenopus (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2K
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE2K/E2-25K Antibody - BSA Free

• E2(25K)
• E225K
• E2-25K
• EC 6.3.2.19
• Hip2
• HIP-2
• huntingtin interacting protein 2
• Huntingtin-interacting protein 2
• HYPG
• LIG
• UbcH1
• UBE2K
• UBE2K-25K
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 K
• Ubiquitin-conjugating enzyme E2(25K)
• Ubiquitin-conjugating enzyme E2-25 kDa
• Ubiquitin-conjugating enzyme E2-25K
• ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
• ubiquitin-protein ligase

Background

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE2K
• Uniprot:
  • P61086(Human)