UBE2K/E2-25K Antibody (701316) [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 701316
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

UBE2K/E2-25K Antibody (701316) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human UBE2K/E2‑25K
  Ala2-Asn200
  Accession # P61086
• Specificity: Detects human UBE2K/E2‑25K in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UBE2K/E2-25K Antibody (701316) [Alexa Fluor® 700]

• E2(25K)
• E225K
• E2-25K
• EC 6.3.2.19
• Hip2
• HIP-2
• huntingtin interacting protein 2
• Huntingtin-interacting protein 2
• HYPG
• LIG
• UbcH1
• UBE2K
• UBE2K-25K
• ubiquitin carrier protein
• ubiquitin-conjugating enzyme E2 K
• Ubiquitin-conjugating enzyme E2(25K)
• Ubiquitin-conjugating enzyme E2-25 kDa
• Ubiquitin-conjugating enzyme E2-25K
• ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
• ubiquitin-protein ligase

Background

UBE2K (Ubiquitin-conjugating enzyme E2; also E2-25K, HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons, monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length. It contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.