![Western Blot: UBE2J2/UBC6 Antibody [NBP2-93049] - Analysis of extracts of various cell lines, using UBE2J2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize2/UBE2J2-UBC6-Antibody-Western-Blot-NBP2-93049-img0003.jpg "Western Blot: UBE2J2/UBC6 Antibody [NBP2-93049] - Analysis of extracts of various cell lines, using UBE2J2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human UBE2J2 (NP_477515.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UBE2J2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/UBE2J1-Antibody-(OTI3D1)---Azide-and-BSA-Free-Western-Blot-NBP2-74747-img0001.jpg)
![Western Blot: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2J1.](http://images.novusbio.com/images2/UBE2J1-Antibody-(OTI3D1)---Azide-and-BSA-Free-Western-Blot-NBP2-74747-img0002.jpg)
![Flow Cytometry: UBE2J1 Antibody (OTI3D1) - Azide and BSA Free [NBP2-74747] - Analysis of Hela cells, using UBE2J1 antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/images2/UBE2J1-Antibody-(OTI3D1)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-74747-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89522-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/images2/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0008.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/images2/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SEC61A Antibody [NBP1-31267] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/images2/SEC61A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31267-img0002.jpg)
![Immunohistochemistry-Paraffin: SEC61A Antibody [NBP1-31267] - Cal27 xenograft, using SEC61A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/SEC61A-Antibody-Immunohistochemistry-Paraffin-NBP1-31267-img0003.jpg)
![Western Blot: SEC61A Antibody [NBP1-31267] - Whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/SEC61A-Antibody-Western-Blot-NBP1-31267-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TXNDC15 Antibody [NBP1-89394] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.](http://images.novusbio.com/images2/TXNDC15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89394-img0004.jpg)
![Immunohistochemistry-Paraffin: TXNDC15 Antibody [NBP1-89394] - Staining of human colon shows cytoplasmic positivity with a granular pattern in glandular cells.](http://images.novusbio.com/images2/TXNDC15-Antibody-Immunohistochemistry-Paraffin-NBP1-89394-img0002.jpg)
![Immunohistochemistry: CD3 delta Antibody (2R1O10) [NBP3-16881] - Immunofluorescence analysis of rat spleen using CD3 delta Rabbit mAb (NBP3-16881) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/CD3-delta-Antibody-(2R1O10)-Immunohistochemistry-NBP3-16881-img0001.jpg)
![Immunohistochemistry: CD3 delta Antibody (2R1O10) [NBP3-16881] - Immunofluorescence analysis of mouse spleen using CD3 delta Rabbit mAb (NBP3-16881) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/CD3-delta-Antibody-(2R1O10)-Immunohistochemistry-NBP3-16881-img0002.jpg)
![Western Blot: CD3 delta Antibody (2R1O10) [NBP3-16881] - Western blot analysis of extracts of Jurkat cells, using CD3 delta Rabbit mAb (NBP3-16881) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/images2/CD3-delta-Antibody-(2R1O10)-Western-Blot-NBP3-16881-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UBE2J2 |
![Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.](http://images.novusbio.com/images/Retinoic-Acid-Receptor-alpha-Antibody-(763)-Western-Blot-NB200-322-img0002.jpg)
![Western Blot: ERp57/PDIA3 Antibody [NBP1-84796] - Analysis using Anti-PDIA3 antibody NBP1-84796 (A) shows similar pattern to independent antibody NBP1-84797 (B).](http://images.novusbio.com/images2/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84796-img0014.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84796 (A) shows similar protein distribution across tissues to independent antibody NBP1-84797 (B).](http://images.novusbio.com/images2/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84796-img0018.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84796] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84796-img0019.jpg)
![UbcH7/UBE2L3 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "UbcH7/UBE2L3 [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")