UBE2J2/UBC6 Antibodies
We offer UBE2J2/UBC6 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each UBE2J2/UBC6 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our UBE2J2/UBC6 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the UBE2J2/UBC6 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our UBE2J2/UBC6 polyclonal antibodies.
Alternate Names for UBE2J2/UBC6 Antibodies
anti-UBE2J2/UBC6 antibody, anti-UBE2J2 antibody, anti-EC 6.3.2.19 antibody, anti-J2 antibody, anti-Ubc6p antibody, anti-ubiquitin-conjugating enzyme E2 J2 antibody, anti-ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) antibody, anti-yeast UBC6 homolog antibody
8 Results for "ube2j2-ubc6" in Primary Antibodies
![Western Blot: UBE2J2/UBC6 Antibody [NBP1-59760] - TMEM129 and Ube2J2 are required for US11-induced FcRn ubiquitination, a, b TMEM129 and Ube2J2 is required for US11-induced FcRn ubiquitination. HeLaFcRn+US11 cells were transfected with 20 nM TMEM129, Ube2J1, or Ube2J2 siRNA oligomers for 48 hr or empty vector. Cells were subsequently treated with 50 uM MG132 for 4 hr and then lysed. All immunoprecipitates or cell lysates (20 ug) as internal controls were subject to western blotting with corresponding Abs as indicated. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-10865-y) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Knockdown-Validated-NBP1-59760-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: UBE2J2/UBC6 Antibody [NBP1-59760] - TMEM129 and Ube2J2 are required for US11-induced FcRn ubiquitination, a, b TMEM129 and Ube2J2 is required for US11-induced FcRn ubiquitination. HeLaFcRn+US11 cells were transfected with 20 nM TMEM129, Ube2J1, or Ube2J2 siRNA oligomers for 48 hr or empty vector. Cells were subsequently treated with 50 uM MG132 for 4 hr and then lysed. All immunoprecipitates or cell lysates (20 ug) as internal controls were subject to western blotting with corresponding Abs as indicated. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-10865-y) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: UBE2J2/UBC6 Antibody [NBP1-59760] - Titration: 1.25ug/ml Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Western-Blot-NBP1-59760-img0003.jpg)
![Immunohistochemistry-Paraffin: UBE2J2/UBC6 Antibody [NBP1-59760] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Immunohistochemistry-Paraffin-NBP1-59760-img0002.jpg)
![Western Blot: UBE2J2/UBC6 Antibody [NBP1-59760] - TMEM129 & Ube2J2 are required for US11-induced FcRn ubiquitination, a, b TMEM129 & Ube2J2 is required for US11-induced FcRn ubiquitination. HeLaFcRn+US11 cells were transfected with 20 nM TMEM129, Ube2J1, or Ube2J2 siRNA oligomers for 48 h or empty vector. Cells were subsequently treated with 50 μM MG132 for 4 h & then lysed. All immunoprecipitates or cell lysates (20 μg) as internal controls were subject to western blotting with corresponding Abs as indicated. c, d HeLaUS11+FcRn cells were transfected with 20 nM Ube2J1 (c, top) & Ube2J2 (c, bottom) siRNA oligomers for 48 h. Cells were then treated with CHX (100 μg/ml) & chased for the indicated time. The cells were lysed after CHX treatment & cell lysates (20 μg) were subject to western blotting with Abs as indicated. The level of remaining FcRn (d) in Ube2j1 siRNA-treated cells (black) or Ube2j2 siRNA-treated cells (red) was quantified as the percentage of the beta -tubulin level at different time points. The percentage of time point 0 (min) is assigned a value of 100% & the values from other time points are normalized to this value. Error bars represented mean ± SEM of three independent repeats Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31289263), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-59760_rabbit-polyclonal-ube2j2-ubc6-antibody-31020241616075.jpg)
![Western Blot: UBE2J2/UBC6 Antibody [NBP1-78071] - expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Western-Blot-NBP1-78071-img0001.jpg "Western Blot: UBE2J2/UBC6 Antibody [NBP1-78071] - expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells.")
![N/A: UBE2J2/UBC6 Antibody [NBP1-78071]](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-N-A-NBP1-78071-img0002.jpg)
![Western Blot: UBE2J2/UBC6 Antibody [NBP2-93049] - Analysis of extracts of various cell lines, using UBE2J2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Western-Blot-NBP2-93049-img0003.jpg "Western Blot: UBE2J2/UBC6 Antibody [NBP2-93049] - Analysis of extracts of various cell lines, using UBE2J2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347] - Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Western-Blot-NBP3-06347-img0001.jpg "Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347] - Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa")
![Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347] - UBE2J1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-UBE2J1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-UBE2J1 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Immunoprecipitation-NBP3-06347-img0002.jpg)
![Western Blot: UBE2J2/UBC6 Antibody [H00118424-B01P] - Analysis of UBE2J2 expression in transfected 293T cell line by UBE2J2 polyclonal antibody. Lane 1: UBE2J2 transfected lysate(28.49 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Western-Blot-H00118424-B01P-img0003.jpg "Western Blot: UBE2J2/UBC6 Antibody [H00118424-B01P] - Analysis of UBE2J2 expression in transfected 293T cell line by UBE2J2 polyclonal antibody. Lane 1: UBE2J2 transfected lysate(28.49 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: UBE2J2/UBC6 Antibody [H00118424-D01P] - Analysis of UBE2J2 expression in transfected 293T cell line by UBE2J2 polyclonal antibody.Lane 1: UBE2J2 transfected lysate(28.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/UBE2J2-UBC6-Antibody-Western-Blot-H00118424-D01P-img0003.jpg "Western Blot: UBE2J2/UBC6 Antibody [H00118424-D01P] - Analysis of UBE2J2 expression in transfected 293T cell line by UBE2J2 polyclonal antibody.Lane 1: UBE2J2 transfected lysate(28.90 KDa).Lane 2: Non-transfected lysate.")