UBE2E3 Antibody

Western Blot: UBE2E3 Antibody [NBP2-20784] - Sample (50 ug of whole cell lysate) A: Mouse Muscle, 12% SDS PAGE gel, diluted at 1:2000.

• Reactivity: Mu, Hu, Rt, Bv, Ch, RM, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

UBE2E3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2E3. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Predicted Species: Human (100%), Rat (100%), Chicken (99%), Xenopus (98%), Zebrafish (95%), Rhesus Macaque (99%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2E3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE2E3 Antibody

• E2-23K
• EC 6.3.2.19
• UBCE4
• UbcH9
• UBCH9ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5)
• UbcM2
• UBE2E3
• Ubiquitin carrier protein E3
• ubiquitin-conjugating enzyme E2 E3
• Ubiquitin-conjugating enzyme E2-23 kDa
• ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase E3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE2E3
• Uniprot:
  • Q969T4(Human)