Recombinant Human UbcH7/UBE2L3 His (N-Term) Protein

• Reactivity: Hu
• Applications: PAGE
• Format: Carrier-Free

Recombinant Human UbcH7/UBE2L3 His (N-Term) Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 154 amino acids corresponding to UbcH7/UBE2L3 and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

  Amino Acid Sequence: MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2L3
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of UbcH7/UBE2L3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
• Preservative: 5% Trehalose
• Purity: >95%, by SDS-PAGE and HPLC

Alternate Names for Recombinant Human UbcH7/UBE2L3 His (N-Term) Protein

• E2-F1
• EC 6.3.2.19
• L-UBC
• UBCE7
• UbcH7
• UBCH7UbcM4
• UbcM4
• UBE2L3
• Ubiquitin carrier protein L3
• ubiquitin-conjugating enzyme E2 L3
• Ubiquitin-conjugating enzyme E2-F1
• ubiquitin-conjugating enzyme E2L 3
• ubiquitin-conjugating enzyme UBCH7
• Ubiquitin-protein ligase L3

Background

Human Ubquitin Conjugating Enzyme E2 L3 (UBE2L3)/UBCH7 is a class I enzyme which functions in the stress response and the control of transcription factors. The enzyme is ubiquitously expressed with high levels of expression seen in adult muscle. UBCH7 mediates the selective degradation of short-lived and abnormal proteins and is highly homologous to UBCH5. It has been demonstrated to participate in the ubiquitinylation of p53, c-Fos and NF-kappaB. UBCH7 is one of two E2s (UBCH5 being the other) with which HECT domain proteins interact with UBCH7 being able to efficiently substitute for UBCH5 in E6-APdependent ubiquitinylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2L3