UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer).

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of Hela lysate (RIPA buffer, 35ug total protein per lane).

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer).

Western Blot: UbcH7/UBE2L3 Antibody [NB300-841] - Analysis of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UbcH7/UBE2L3 Antibody Summary

• Description: Novus Biologicals Goat UbcH7/UBE2L3 Antibody (NB300-841) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EFTKKYGEKRPVD corresponding to C-Terminus according to NP_003338.1.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBE2L3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 18 kDa band observed in HeLa, HEK293, HepG2, and Jurkat lysates, in human, mouse, and rat heart, skeletal muscle, and testis lysates, and in porcine heart lysate (calculated MW of 17.9 kDa band according to human NP_003338.1 and mouse NP_033482.1).
• Theoretical MW: 17.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  UbcH7/UBE2L3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UbcH7/UBE2L3 Antibody

• E2-F1
• EC 6.3.2.19
• L-UBC
• UBCE7
• UbcH7
• UBCH7UbcM4
• UbcM4
• UBE2L3
• Ubiquitin carrier protein L3
• ubiquitin-conjugating enzyme E2 L3
• Ubiquitin-conjugating enzyme E2-F1
• ubiquitin-conjugating enzyme E2L 3
• ubiquitin-conjugating enzyme UBCH7
• Ubiquitin-protein ligase L3

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). Ube2L3 gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Ubiquitous, with highest expression in testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2L3
• Entrez:
  • 7332(Human)
  • 22195(Mouse)
• Uniprot:
  • NP_005330.(Human)