Recombinant Human UbcH5c/UBE2D3 Protein

Product Discontinued

Recombinant Human UbcH5c/UBE2D3 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-149 of Human UbcH5c/UBE2D3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLSNRKCLSK ELSDLARDPP AQCSAGPVGD DMFHWQATIM GPNDSPYQGG VFFLTIHFPT DYPFKPPKVA FTTRIYHPNI NSNGSICLDI LRSQWSPALT ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDRDKYNRIS REWTQKYAM

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2D3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human UbcH5c/UBE2D3 Protein

• E2(17)KB 3
• E2(17)KB3
• EC 6.3.2.19
• MGC43926
• MGC5416
• UBC4/5
• UBC5C
• UbcH5c
• UBE2D3
• Ubiquitin carrier protein D3
• ubiquitin-conjugating enzyme E2 D3
• Ubiquitin-conjugating enzyme E2(17)KB 3
• Ubiquitin-conjugating enzyme E2-17 kDa 3
• ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
• ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D3

Background

UBE2D3, also known as ubiquitin-conjugating enzyme E2 D3. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Recombinant human UBE2D3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2D3