UbcH5a/UBE2D1 Antibody (2C6) - Azide and BSA Free

Western Blot: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01] - Analysis of UBE2D1 expression in transfected 293T cell line by UBE2D1 monoclonal antibody (M01), clone 2C6.Lane 1: UBE2D1 transfected lysate(16.6 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01] - Analysis of monoclonal antibody to UBE2D1 on formalin-fixed paraffin-embedded human cervix cancer. Antibody concentration 1 ug/ml.

Sandwich ELISA: UbcH5a/UBE2D1 Antibody (2C6) [H00007321-M01] - Detection limit for recombinant GST tagged UBE2D1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UbcH5a/UBE2D1 Antibody (2C6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: UBE2D1 (NP_003329, 1 a.a. ~ 94 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWS
• Specificity: UBE2D1 (2C6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE2D1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UbcH5a/UBE2D1 Antibody (2C6) - Azide and BSA Free

• E2(17)KB1
• Stimulator of Fe transportUBC4/5 homolog
• UBC4/5
• UBC5A
• UbcH5
• UbcH5a
• UBCH5AEC 6.3.2.19
• UBCH5SFT
• UBE2D1
• Ubiquitin carrier protein D1
• ubiquitin-conjugating enzyme E2 D1
• Ubiquitin-conjugating enzyme E2(17)KB 1
• Ubiquitin-conjugating enzyme E2-17 kDa 1
• ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase D1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UbcH5a/UBE2D1 Antibody (H00007321-M01)(1)

Publication using H00007321-M01

• Zhu Z, Du T, Zhou G, Roizman B. The stability of HSV-1 ICP0 early after infection is defined by the RING finger and the UL13 protein kinase. J Virol. 2014-01-10 [PMID: 24574411]

Bioinformatics

• Gene Symbol: UBE2D1
• Entrez:
  • 7321(Human)
• OMIM:
  • 602961(Human)
• Uniprot:
  • NP_003329(Human)