Recombinant Human UbcH10/UBE2C Protein

Product Discontinued

Recombinant Human UbcH10/UBE2C Protein Summary

• Description: Recombinant protein for Human UbcH10/UBE2C
  
  

  Source:E. coli
  Amino Acid Sequence:(P61086)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2C
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 20 mM TBS, pH 7.5 containing 1.0 mM DTT.
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human UbcH10/UBE2C Protein

• cyclin-selective ubiquitin carrier protein
• dJ447F3.2
• EC 6.3.2.19
• UbcH10
• UBCH10mitotic-specific ubiquitin-conjugating enzyme
• UBE2C
• Ubiquitin carrier protein C
• ubiquitin carrier protein E2-C
• ubiquitin-conjugating enzyme E2 C
• ubiquitin-conjugating enzyme E2C
• Ubiquitin-protein ligase C

Background

Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1uM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2C