TSP50 Antibody

Western Blot: TSP50 Antibody [17190002] - Samples: Lane 1, Marker [kDa]: 219, 111, 83, 48, 32, 26, 17 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight [kDa]: 43 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: TSP50 Antibody [17190002] - Strong cytoplasmic positivity was seen in primary spermatocytes of the seminiferous tubules of the testes, neurons of the cerebral cortex and many hematopoeitic cells of the bone marrow. Most mucosal surfaces and glands showed moderate or some times weak positivity. Biliary ductal cells and gall bladder mucosal cells showed strong accentuation of the luminal cell membrane. Most other normal tissues showed moderate or weak positivity. More consistent and stronger expression was seen in malignancies. Strong or moderate positivity was seen in prostatic, testicular and colo-rectal cancers. Many pancreatic, prostatic and some testicular cancers showed strong cytoplasmic granularity. Strong or moderate positivity was also seen in malignant carcinoid, renal and urothelial cancers. Most other cancers showed moderate or sometimes weak positivity. Image and statement courtesy of the Human Protein Atlas (HPA).

ELISA: TSP50 Antibody [17190002]

Product Discontinued

TSP50 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein.
• Specificity: This product is specific for Human TSP50.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRSS50
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:22844329).

Reactivity Notes

Human and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for TSP50 Antibody

• Cancer/testis antigen 20
• CT20probable threonine protease PRSS50
• EC 3.4.25.-
• protease, serine, 50
• Serine protease 50
• testes-specific protease 50
• Testis-specific protease-like protein 50
• TSP50
• TSP50testes specific protease 50

Background

TSP50 is a possible protease in human testes. It may function in mammalian spermatogenesis. Its linear catalytic structure is similar to many serine proteases, except for a Thr-for-Ser residual catalytic site substitution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSP50 Antibody (17190002)(3)

Publications using 17190002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.
• Cheng CW, Chang WL, Chang LC et al. Ferulic Acid, an Angelica sinensis-Derived Polyphenol, Slows the Progression of Membranous Nephropathy in a Mouse Model Evid Based Complement Alternat Med 2012-01-01 [PMID: 22844329] (IHC-Fr)

Bioinformatics

• Gene Symbol: PRSS50
• Uniprot:
  • NP_037402(Human)