TRRAP Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

TRRAP Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human TRRAP
  Asn3610-Leu3859, predicted
  Accession # Q9Y4A5
• Specificity: Detects human, mouse and rat TRRAP in Western blots. Detects recombinant human TRRAP in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRRAP Antibody [Alexa Fluor® 594]

• 350/400 kDa PCAF-associated factor
• FLJ10671
• PAF350/400
• PAF400
• PAF400STAF40
• STAF40
• Tra1 homolog
• Tra1
• transformation/transcription domain-associated protein
• TR-AP
• TRRAP

Background

TRRAP (Transformation/transcription domain-associated protein; also PAF350/400 and STAF40) is a 400-450 kDa member of the TRA1 subfamily, PI3/PI4 kinase family, ATM superfamily of molecules. It is a ubiquitously expressed nuclear protein that functions as a promoter of gene transcription. In particular, the activity of the E2F transcription factor family is regulated by the degree of histone acetylation. Deacetylation inhibits E2F-mediated transcription, while acetylation promotes gene transcription. TRRAP promotes histone acetylation by serving as a positive modulator/cofactor for GCN5 acetyltransferase activity. TRRAP also acts independently of GCN5. It serves as a recruiter for Skp1, a ubiquitin ligase that ubiquitates chromatin-bound beta -catenin, leading to a downregulation of Wnt-signaling activity. Human TRRAP is 3859 amino acids (aa) in length. It contains one Pro-rich region (aa 485-526), an NLS (aa 2047-2062), two FAT domains (aa 2704-3275 and 3827-3859) and a nonenzymatic PI3/PI4 kinase domain (aa 3528-3826). There are multiple potential isoform variants. They show various combinations of a deletion involving aa 1493-1510, an Ala substitution for aa 3001-3012, a 119 aa substitution for aa 3784-3859, and an 18 aa insertion after Asp3287. Over aa 3610-3859, human TRRAP shares 99% aa identity with mouse TRRAP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.