Troponin I Type 1 (slow skeletal) Antibody (7G2) [Janelia Fluor® 646]

• Reactivity: Hu, In
• Applications: WB, ELISA, AP
• Clone: 7G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

Troponin I Type 1 (slow skeletal) Antibody (7G2) [Janelia Fluor® 646] Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human skTnI.
• Specificity: Troponin I from human skeletal muscle.Cross-reaction of this with cardiac troponin I is less then 1.5%.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Immunoassay
  • Sandwich ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 26213227).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Troponin I Type 1 (slow skeletal) Antibody (7G2) [Janelia Fluor® 646]

• DKFZp451O223
• slow-twitch isoform
• SSTNI
• TNN1
• troponin I type 1 (skeletal, slow)
• troponin I, skeletal, slow
• troponin I, slow skeletal muscle

Background

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI1