Tropomyosin-1 Antibody (TM311)

Immunocytochemistry/Immunofluorescence: Tropomyosin-1 Antibody (TM311) [NB100-1908] - Localization of microfilament associated proteins in PTK2 cells using (1) Monoclonal Anti-Tropomyosin, (2) Monoclonal Anti-Myosin (Light Chain), and (3) Monoclonal Anti-alpha-Actinin

Immunocytochemistry/Immunofluorescence: Tropomyosin-1 Antibody (TM311) [NB100-1908] - A formaldehyde-fixed cardiomyocyte isolated from rat heart using Monoclonal Anti-Tropomyosin (36/39 kDa) produced in mouse

Product Discontinued

Tropomyosin-1 Antibody (TM311) Summary

• Immunogen: Chicken gizzard tropomyosin.
• Localization: Cytoplasmic
• Specificity: This is specific for the 36K and 39K bands of chicken gizzard tropomyosin. This stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPM1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID: 23918370)
• Control:

  Tropomyosin-1 Overexpression Lysate

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Tropomyosin-1 Antibody (TM311)

• alpha tropomyosin
• alpha-tropomyosin
• C15orf13
• cardiomyopathy, hypertrophic 3
• chromosome 15 open reading frame 13
• CMD1Y
• CMH3
• HTM-alpha
• sarcomeric tropomyosin kappa
• TMSA
• tropomyosin 1 (alpha) isoform 1
• tropomyosin 1 (alpha) isoform 2
• tropomyosin 1 (alpha) isoform 3
• tropomyosin 1 (alpha) isoform 4
• tropomyosin 1 (alpha) isoform 5
• tropomyosin 1 (alpha) isoform 6
• tropomyosin 1 (alpha) isoform 7
• tropomyosin 1 (alpha)
• tropomyosin alpha-1 chain
• tropomyosin-1

Background

Tropomyosin is a rigid rod shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tropomyosin-1 Antibody (NB100-1908)(4)

Publications using NB100-1908

• Colpan M, Iwanski J, Gregorio CC CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle Communications biology 2021-03-19 [PMID: 33742108] (WB, Mouse, Rat)
• Ahrens-Nicklas RC, Pappas CT, Farman GP, et al Disruption of cardiac thin filament assembly arising from a mutation in LMOD2: A novel mechanism of neonatal dilated cardiomyopathy. Sci Adv. 2019-09-04 [PMID: 31517052] (WB, Human)
• Hu J, Ho AL, Yuan L et al. Neutralization of terminal differentiation in gliomagenesis. Proc Natl Acad Sci U S A 2013-08-05 [PMID: 23918370] (IF/IHC, Mouse)
• Gao H, Steffen MC, Ramos KS. Osteopontin Regulates [alpha]-Smooth Muscle Actin and Calponin in Vascular Smooth Muscle Cells. Cell Biology International. 2011-10-28 [PMID: 22032345]

FAQs for Tropomyosin-1 Antibody (NB100-1908). (Showing 1 - 1 of 1 FAQs).

1. I am looking for a mouse anti human TPM1 antibody, and am interested in NB100-1908. However, I specifically need one with cross reactivity against all TPM1 isoforms. Could you tell me what epitope your antibody recognizes, or failing that (I realize this is often confidential), which exon of the gene the epitope is situated in? I could not find this information on the datasheet, and therefore cannot tell whether your antibody would suit the application I have in mind.
   • In regards to your inquiry, unfortunately NB100-1908 has not been epitope mapped so we are unaware of the location or exon that the epitope is situated in. I am not sure what application and species you were looking for an antibody against, but this TPM1 antibody (NBP1-89896) for example has the immunogen sequence listed for you and might be helpful since you will have access to this information.

Bioinformatics

• Gene Symbol: TPM1
• Entrez:
  • 396366(Chicken)
  • 7168(Human)
  • 22003(Mouse)
  • 100125989(Rabbit)
  • 24851(Rat)
• Uniprot:
  • P04268(Chicken)