Tropomyosin-1 Antibody (TM311)

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Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody (TM311) [NB100-1908] - Localization of microfilament associated proteins in PTK2 cells using (1) Monoclonal Anti-Tropomyosin, (2) Monoclonal Anti-Myosin ...read more
Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody (TM311) [NB100-1908] - A formaldehyde-fixed cardiomyocyte isolated from rat heart using Monoclonal Anti-Tropomyosin (36/39 kDa) produced in mouse

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, MiAr
Clone
TM311
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Tropomyosin-1 Antibody (TM311) Summary

Immunogen
Chicken gizzard tropomyosin.
Localization
Cytoplasmic
Specificity
This is specific for the 36K and 39K bands of chicken gizzard tropomyosin. This stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TPM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:400
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 23918370)
Positive Control
Tropomyosin-1 Lysate (NBL1-17219)
Tropomyosin-1 Lysate (NBP2-65257)
Publications
Read Publications using
NB100-1908 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Tropomyosin-1 Antibody (TM311)

  • alpha tropomyosin
  • alpha-tropomyosin
  • C15orf13
  • cardiomyopathy, hypertrophic 3
  • chromosome 15 open reading frame 13
  • CMD1Y
  • CMH3
  • HTM-alpha
  • sarcomeric tropomyosin kappa
  • TMSA
  • tropomyosin 1 (alpha) isoform 1
  • tropomyosin 1 (alpha) isoform 2
  • tropomyosin 1 (alpha) isoform 3
  • tropomyosin 1 (alpha) isoform 4
  • tropomyosin 1 (alpha) isoform 5
  • tropomyosin 1 (alpha) isoform 6
  • tropomyosin 1 (alpha) isoform 7
  • tropomyosin 1 (alpha)
  • tropomyosin alpha-1 chain
  • tropomyosin-1

Background

Tropomyosin is a rigid rod shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tropomyosin-1 Antibody (NB100-1908)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tropomyosin-1 Antibody (NB100-1908). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for a mouse anti human TPM1 antibody, and am interested in NB100-1908. However, I specifically need one with cross reactivity against all TPM1 isoforms. Could you tell me what epitope your antibody recognizes, or failing that (I realize this is often confidential), which exon of the gene the epitope is situated in? I could not find this information on the datasheet, and therefore cannot tell whether your antibody would suit the application I have in mind.
    • In regards to your inquiry, unfortunately NB100-1908 has not been epitope mapped so we are unaware of the location or exon that the epitope is situated in. I am not sure what application and species you were looking for an antibody against, but this TPM1 antibody (NBP1-89896) for example has the immunogen sequence listed for you and might be helpful since you will have access to this information.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPM1
Entrez
Uniprot