Tripeptidyl-Peptidase I/TPP1 Antibody (254008)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 254008
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Tripeptidyl-Peptidase I/TPP1 Antibody (254008) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Tripeptidyl‑Peptidase I/TPP1
  Ser20-Pro563
  Accession # O14773
• Specificity: Detects human Tripeptidyl‑Peptidase I/TPP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tripeptidyl-Peptidase I/TPP1 Antibody (254008)

• Cell growth-inhibiting gene 1 protein
• ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease)
• CLN2
• CLN2EC 3.4.14.9
• growth-inhibiting protein 1
• LINCL
• LPIC
• lysosomal pepstatin insensitive protease
• Lysosomal pepstatin-insensitive protease
• MGC21297
• TPP1
• TPP-1
• TPP-I
• Tripeptidyl aminopeptidase
• tripeptidyl peptidase I
• tripeptidyl-peptidase 1
• TripeptidylPeptidase I
• Tripeptidyl-Peptidase I

Background

Tripeptidyl-Peptidase I (TPP1), also known as tripeptidyl aminopeptidase, is a lysosomal peptidase which can hydrolyze tripeptides from the N-termini of oligopeptides and also possesses weak endopeptidase activity (1‑3). TPP1 is a serine peptidase with a Ser-Glu-Asp catalytic triad, making it a member of the sedolisin family (4). The TPP1 precursor undergoes autoactivation under conditions of acidic pH (4). TPP1 is expressed in many tissues, with elevated expression in tissues associated with peptide hormone production (5). Mutations in TPP1 have been shown to be a cause of classic late‑infantile neuronal ceroid lipofuscinosis (CLN2), a lysosomal storage disease (6).

1. Sleat, D.E. et al. (1997) Science 277:1802.
2. Ezaki, J. et al. (1999) J. Neurochem. 72:2573.
3. Tomkinson, B. (1999) Trends Biochem. Sci. 24:355.
4. Lin, L. et al. (2001) J. Biol. Chem. 276:2249.
5. Kida, E. et al. (2001) J. Neuropathol. Exp. Neurol. 60:280.
6. Sleat, D.E. et al. (1999) Am. J. Hum. Genet. 64:1511.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tripeptidyl-Peptidase I/TPP1 Antibody (MAB2237)(1)

Publication using MAB2237

• Nicholas Buss, Lisa Lanigan, Jillynne Zeller, Derek Cissell, Monica Metea, Eric Adams et al. Characterization of AAV-mediated dorsal root ganglionopathy Molecular Therapy - Methods & Clinical Development 2022-03-10 [PMID: 35229008]

Bioinformatics

• Gene Symbol: TPP1
• Uniprot:
  • O14773()