TRIM29 Antibody

Western Blot: TRIM29 Antibody [NBP1-52000] - (1ug/ml) staining of Peripheral Blood Mononucleocyte (A) and (0.1 ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Intermediate filament staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-52000] - (3.8ug/ml) staining of Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM29 Antibody Summary

• Description: Novus Biologicals Goat TRIM29 Antibody (NBP1-52000) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-TRIM29 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKDRIKSFTTNE corresponding to internal region according to NP_036233.2.
• Epitope: C-EKDRIKSFTTNE
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM29
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1-1 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 65.8 kDa band according to NP_036233.2). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room tempetature.
  IHC: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes.
  Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filament of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells.
  Flow Cytometry: Flow cytometric analysis of A431 cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM29 Antibody

• Ataxia telangiectasia group D-associated protein
• ATDC ataxia-telangiectasia group D-associated protein
• ATDC
• FLJ36085
• tripartite motif containing 29
• tripartite motif protein TRIM29
• tripartite motif-containing 29
• tripartite motif-containing protein 29

Background

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM29 Antibody (NBP1-52000)(1)

Publication using NBP1-52000

• Wang L, Heidt DG, Lee CJ et al. Oncogenic function of ATDC in pancreatic cancer through Wnt pathway activation and beta-catenin stabilization. Cancer Cell 2009-03-01 [PMID: 19249679]

Bioinformatics

• Gene Symbol: TRIM29
• Entrez:
  • 23650(Human)
  • 72169(Mouse)
  • 300656(Rat)
• Uniprot:
  • NP_036233.2(Human)