Trim11 Antibody

Images

 
Western Blot: Trim11 Antibody [NB100-1214] - Trim11 in human D54 malignant glioma cell line. Image provided by Kaijun Di.
Immunohistochemistry-Paraffin: Trim11 Antibody [NB100-1214] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Trim11 Antibody Summary

Immunogen
Peptide with sequence CRPKGGSGDTLAPQ corresponding to C-Terminus according to NP_660215.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments in human heart, kidney, liver and lung lysates gave no specific signal but low background. IHC-P: Human kidney shows cytoplasm staining preferentially in PCT over DCT. BTrim11 antibody validated for WB from a verified customer review.
Control
Trim11 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-1214 in the following applications:

Publications
Read Publication using NB100-1214.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Trim11 Antibody

  • BIA1
  • EC 6.3.2.-
  • Protein BIA1
  • RING finger protein 92
  • RNF92
  • RNF92E3 ubiquitin-protein ligase TRIM11
  • TRIM11
  • tripartite motif containing 11
  • tripartite motif-containing 11
  • Tripartite motif-containing protein 11

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Trim11 Antibody (NB100-1214)(1)


Review for Trim11 Antibody (NB100-1214) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1214:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Trim11 NB100-1214
Enlarge
reviewed by:
Anonymous
WB Human 02/21/2013
View

Summary

ApplicationWestern Blot
Sample Testedhuman D54 malignant glioma cell line
SpeciesHuman
LotP1
Commentshigh background. need enough wash.

Blocking

Blocking Details10% non-fat milk in TBST for 1 hr at room temperature

Primary Anitbody

Dilution Ratio1:4000 dilution with 1%BSA in PBS overnight at 4 degree

Secondary Antibody

Secondary Descriptionrabbit anti goat IgG, HRP
Secondary Manufacturer Cat#Chemicon, Cat# AP106P
Secondary Concentration1;15000

Details

Detection NotesAmersham ECL Prime Western Blotting Detection Reagent, film exposure

Comments

Commentshigh background. need enough wash.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Trim11 Antibody (NB100-1214) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Visit Tool

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Blogs on Trim11

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Contact Information

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Recent Reviews

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Anonymous
02/21/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol TRIM11
Entrez
Uniprot