Trim11 Antibody

Western Blot: Trim11 Antibody [NB100-1214] - Trim11 in human D54 malignant glioma cell line. Image provided by Kaijun Di.

Immunohistochemistry-Paraffin: Trim11 Antibody [NB100-1214] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Trim11 Antibody Summary

• Immunogen: Peptide with sequence CRPKGGSGDTLAPQ corresponding to C-Terminus according to NP_660215.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments in human heart, kidney, liver and lung lysates gave no specific signal but low background. IHC-P: Human kidney shows cytoplasm staining preferentially in PCT over DCT. BTrim11 antibody validated for WB from a verified customer review.
• Control:

  Trim11 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Trim11 Antibody

• BIA1
• EC 6.3.2.-
• Protein BIA1
• RING finger protein 92
• RNF92
• RNF92E3 ubiquitin-protein ligase TRIM11
• TRIM11
• tripartite motif containing 11
• tripartite motif-containing 11
• Tripartite motif-containing protein 11

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trim11 Antibody (NB100-1214)(1)

Publication using NB100-1214

• Reymond A, Meroni G, Fantozzi A et al. The tripartite motif family identifies cell compartments. EMBO J 2001-05-01 [PMID: 11331580]

Review for Trim11 Antibody (NB100-1214) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kaijun Di WB Human 02/21/2013

Summary

• Application: Western Blot
• Sample Tested: human D54 malignant glioma cell line
• Species: Human
• Lot: P1

Bioinformatics

• Gene Symbol: TRIM11
• Entrez:
  • 81559(Human)
• Uniprot:
  • NP_660215()