Trim11 Overexpression Lysate (Native)

Images

 
Western Blot: Trim11 Overexpression Lysate (Native) [NBL1-17275] - analysis of Trim 11 expression. Image from verified customer review.
Western Blot: Trim11 Overexpression Lysate (Adult Normal) [NBL1-17275] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Trim11.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Trim11 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209630 and based on accession number NM_145214. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tripartite motif-containing 11 (TRIM11), mRNA.
Gene
TRIM11

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBL1-17275 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Trim11

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Trim11 Overexpression Lysate (Native)

  • BIA1
  • EC 6.3.2.-
  • Protein BIA1
  • RING finger protein 92
  • RNF92E3 ubiquitin-protein ligase TRIM11
  • tripartite motif containing 11
  • tripartite motif-containing 11
  • Tripartite motif-containing protein 11

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nucleus and the cytoplasm. Its function has not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Trim11 Lysate (NBL1-17275) (0)

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Review for Trim11 Lysate (NBL1-17275) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-17275:
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Images Ratings Applications Species Date Details
Western Blot Trim11 NBL1-17275
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reviewed by:
Kaijun Di
WB Human 10/03/2014
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Summary

ApplicationWestern Blot
Sample TestedD54 glioma cell
SpeciesHuman

Blocking

Blocking Details10% milk in TBST

Primary Anitbody

Dilution RatioNB100-1214 1:4000 in 1% BSA of TBST, 4 degree overnight

Secondary Antibody

Secondary Descriptionrabbit anti-goat IgG, HRP
Secondary Manufacturer Cat#AP106P
Secondary Concentration1:15000

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Video Protocols

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FAQs for Trim11 Lysate (NBL1-17275) (0)

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Kaijun Di
10/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TRIM11