Trichohyalin Antibody (AE15)

Product Discontinued

Trichohyalin Antibody (AE15) Summary

• Immunogen: Urea-mercaptoethanol extract of human hair follicles
• Specificity: AE15 recognizes a 220K trichohyalin granular protein, a marker for hair follicular differentiation
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCHH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Trichohyalin Antibody (AE15)

• THL
• TRHY
• trichohyalin

Background

Trichohyalin is a hair follicle protein with characteristics similar to those of profilaggrin (135940), involucrin (147360), and loricrin (152445). Trichohyalin is incorporated into the IF (intermediate filament)-like structures in the mature cells of the hair follicle's inner root sheath. Like profilaggrin, involucrin, and loricrin, trichohyalin becomes highly cross linked by the enzyme transglutaminase. The trichohyalin molecule has a mass of approximately 200 kD and consists predominantly of tandem repeats of an approximately 23-amino acid sequence. Fietz et al. (1992) used a sheep trichohyalin cDNA probe in tritium-labeled in situ hybridization to demonstrate that the human THL gene is located in the region 1q21.1-q23, probably 1q21.3. Profilaggrin, involucrin, and loricrin map to the same area, suggesting that the 4 proteins form a novel superfamily of epithelial structural proteins. By analysis of human-rodent somatic cell hybrids and fluorescence in situ hybridization, Lee et al. (1993) confirmed the mapping of THL to 1q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCHH
• Entrez:
  • 7062(Human)