TRIB2 Antibody (OTI8D11)

Western Blot: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB2 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of COS7 cells using anti-TRIB2 mouse monoclonal antibody .

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Human endometrium tissue using anti-TRIB2 mouse monoclonal antibody.

Flow Cytometry: TRIB2 Antibody (OTI8D11) [NBP1-48030] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIB2 antibody, and then analyzed by flow cytometry.

Western Blot: TRIB2 Antibody (OTI8D11) [NBP1-48030] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB2.

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRIB2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TRIB2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRIB2 Antibody (OTI8D11) [NBP1-48030] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIB2 mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI8D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TRIB2 Antibody (OTI8D11) Summary

• Description: Novus Biologicals Mouse TRIB2 Antibody (OTI8D11) (NBP1-48030) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TRIB2 (NP_067675) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
• Theoretical MW: 38.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIB2 Antibody (OTI8D11)

• C5FW
• FLJ57420
• GS3955
• TRB-2
• TRB2tribbles homolog 2
• tribbles homolog 2 (Drosophila)

Background

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIB2
• Entrez:
  • 28951(Human)