TR beta 1/NR1A2/Thyroid Hormone Receptor beta Overexpression Lysate

Western Blot: Thyroid Hormone Receptor beta Overexpression Lysate (Adult Normal) [NBL1-16896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Thyroid Hormone Receptor beta.

• Reactivity: Hu
• Applications: WB

TR beta 1/NR1A2/Thyroid Hormone Receptor beta Overexpression Lysate Summary

• Description:

  TR beta 1/NR1A2/Thyroid Hormone Receptor beta Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC217873
  
  Accession#: NM_000461
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: THRB

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 52.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for TR beta 1/NR1A2/Thyroid Hormone Receptor beta Overexpression Lysate

• avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2
• c-erbA-2
• c-erbA-beta
• ERBA2MGC126109
• ERBA-BETA
• GRTH
• MGC126110
• NR1A2
• NR1A2thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog 2)
• Nuclear receptor subfamily 1 group A member 2
• oncogene ERBA2
• PRTH
• THR1pituitary resistance to thyroid hormone
• THRB
• THRB1
• THRB2
• thyroid hormone receptor beta 1
• thyroid hormone receptor beta
• thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a)oncogene homolog 2, avian)
• TR beta 1
• v-ErbA2

Background

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the predominant transcript. Transcript variants 1-3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: THRB