TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody

Product Discontinued

TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody Summary

• Immunogen: Synthetic peptide: EVFEDQEV, corresponding to amino acids 403-410 of Human Thyroid Hormone Receptor alpha 1.
• Localization: Nuclear
• Specificity: This does not detect TRv alpha -2 or TR beta-1.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THRA
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: GSA: Use at an assay dependent dilution. ICC: Use at a dilution of 1/200. Western Blot: Use at a dilution of 1/200. Detects a band of approximately 48 kDa (predicted molecular weight: 59 kDa) representing recombinant human TR alpha-1 expressed in E. coli. Gel shift experiments show that this product reacts with TR alpha-1 homodimers and TR alpha-1/retinoic X receptor (RXR) heterodimers. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Chicken and Human. Expected to cross-react with Pig (100% identity with immunogen), Sheep (100% identity with immunogen), Xenopus laevis (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody

• AR7
• c-ERBA-1
• c-erbA-alpha
• EAR7
• EAR-7
• EAR-7.1/EAR-7.2
• ERBA
• ERBA1thyroid hormone receptor, alpha (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog)
• ERBA-related 7
• MGC000261
• MGC43240
• NR1A 1
• NR1A1
• NR1A1THRA3
• Nuclear receptor subfamily 1 group A member 1
• THRA
• THRA1ERB-T-1
• THRA2
• thyroid hormone receptor alpha
• thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a)oncogene homolog, avian)
• TR alpha
• triiodothyronine receptor
• v-ErbA1
• V-erbA-related protein 7

Background

Thyroid hormone receptors (TR) are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THRA