TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody

Product Discontinued

TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acids 13-32 of Human Thyroid Hormone Receptor alpha 1.
• Localization: Nuclear
• Specificity: This reacts with both Thyroid Hormone Receptor alpha 1 and Thyroid Hormone Receptor alpha 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC-P: Use at a dilution of 1/50 - 1/100. Staining of formalin fixed tissues requires high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Suggested incubation period is 30 minutes at room temperature.

Reactivity Notes

Cross-reacts with Human, Rat and Pig. May cross react with Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody

• AR7
• c-ERBA-1
• c-erbA-alpha
• EAR7
• EAR-7
• EAR-7.1/EAR-7.2
• ERBA
• ERBA1thyroid hormone receptor, alpha (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog)
• ERBA-related 7
• MGC000261
• MGC43240
• NR1A 1
• NR1A1
• NR1A1THRA3
• Nuclear receptor subfamily 1 group A member 1
• THRA
• THRA1ERB-T-1
• THRA2
• thyroid hormone receptor alpha
• thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a)oncogene homolog, avian)
• TR alpha
• triiodothyronine receptor
• v-ErbA1
• V-erbA-related protein 7

Background

Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR Beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3' -triiodo-L-thyronine (T-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THRA
• Entrez:
  • 81812(Rat)