TPMT Antibody

Product Discontinued

TPMT Antibody Summary

• Immunogen: Synthetic peptide: C-DAFEERHKSWGID, representing the C Terminus of the human protein according to NP_000358.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:16000
• Application Notes: Western blot: Preliminary experiments in Human Bone Marrow, Kidney, Liver and Testis lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
• Control:

  TPMT Overexpression Lysate

Reactivity Notes

Human. Expected to react based Mouse, Rat, Dog, Cow

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for TPMT Antibody

• EC 2.1.1.67
• S-adenosyl-L-methionine:thiopurine S-methyltransferase
• Thiopurine methyltransferase
• thiopurine S-methyltransferase
• TPMT

Background

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPMT
• Entrez:
  • 7172(Human)
  • 22017(Mouse)
  • 690050(Rat)
• Uniprot:
  • NP_000358.1(Human)