TOR/mTOR Antibody (6611)

Images

 
Genetic Strategies: Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: TOR/mTOR Antibody (6611) [NBP2-43658] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: mTOR protein stained by mTOR antibody [6611] diluted at 1:100. Blue: ...read more
Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A Jurkat whole cell lysate/extract B Raji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:1000.
Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Sh, ZeSpecies Glossary
Applications WB, ICC/IF
Clone
6611
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

TOR/mTOR Antibody (6611) Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (99%), Sheep (98%), Zebrafish (90%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MTOR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
289 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for TOR/mTOR Antibody (6611)

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TOR/mTOR Antibody (NBP2-43658) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TOR/mTOR Antibody (NBP2-43658)

Discover related pathways, diseases and genes to TOR/mTOR Antibody (NBP2-43658). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TOR/mTOR Antibody (NBP2-43658)

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Pathways for TOR/mTOR Antibody (NBP2-43658)

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PTMs for TOR/mTOR Antibody (NBP2-43658)

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Research Areas for TOR/mTOR Antibody (NBP2-43658)

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Blogs on TOR/mTOR.

Staufen1 Overabundance and the Consequent mTOR Hyperactivity in Amyotrophic Lateral Sclerosis, Spinocerebellar Ataxia Type 2, Alzheimer’s, Parkinson’s, and Huntington’s Diseases
Jamshed Arslan, Pharm D, PhD Neurodegenerative disorders involve loss of function and, ultimately, death of neurons. Selective neuronal vulnerability has been observed in a variety of neurodegenerative diseases. Fo...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

The Many Connections Between Autophagy and Kidney Disease
By Yoskaly Lazo-Fernandez, PhD The first description of what is called today an autophagosome was given in a paper published in 1957. Its author employed electron microscopy to observe the neonatal features of mous...  Read full blog post.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol MTOR