TOP1MT Antibody (TOP1MT/2883R)

Images

 
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/2883R) [NBP3-07568] - Formalin-fixed, paraffin-embedded human Pancreas stained with TOP1MT Rabbit Recombinant Monoclonal Antibody (TOP1MT/2883R).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
TOP1MT/2883R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

TOP1MT Antibody (TOP1MT/2883R) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08332)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant full-length human TOP1MT protein (Uniprot: Q969P6)
Localization
Cytoplasmic (Mitochondria)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TOP1MT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TOP1MT Antibody (TOP1MT/2883R)

  • mitochondrial DNA topoisomerase I
  • mitochondrial
  • topoisomerase (DNA) I, mitochondrial

Background

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TOP1MT
Uniprot