TNN Antibody (MTn-12)

Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210] - Frozen sections, acetone fixed of mouse intestine were stained with 1:400 monoclonal TNN Antibody (Clone: MTn-12) followed by 1:20 monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse and by 1:20 Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat.

• Reactivity: Mu
• Applications: WB, DB, ELISA, IHC, IP
• Clone: MTn-12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TNN Antibody (MTn-12) Summary

• Immunogen: Partially purified mouse tenascin.
• Localization: In immunohistological testing of frozen tissue sections of mouse intestine, the antibody stains the core of the villi but not the epithelial cells.
• Specificity: This antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of approximate molecular weights 210 and 260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblasts lysates under reducing conditions.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TNN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: IHC-Fr: In immunohistological testing of frozen tissue sections of mouse intestine, using immunofluorescent or immunoperoxidase staining, the antibody stains the core of the villi but not the epithelial cells. Dilution: 1:200.
  ELISA: In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I.

Reactivity Notes

. No cross reactivity with tenascin of other species has been observed.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for TNN Antibody (MTn-12)

• tenascin N
• tenascin-N
• tenascin-W
• TN-N
• TNW
• TN-W

Background

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors (Erickson and Bourdon 1989).It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).The tenascin molecule is a disulfide-linked hexamer.The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNN