TNIP2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

TNIP2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human TNIP2.
  
  

  Source: E. coli

  Amino Acid Sequence: MLEQQILAYKDDFMSERADRERAQSRIQELEEKVASLLHQVSWRQDSREPDAGRIHAGSKTAKYLAADALELMVPGGWRP

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: TNIP2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-48949.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for TNIP2 Recombinant Protein Antigen

• A20-binding inhibitor of NF-kappa-B activation 2
• A20-binding inhibitor of NF-kappaB activation-2
• ABIN-2
• ABIN2KLIP
• Fetal liver LKB1-interacting protein
• FLIP1DKFZp434J1313
• MGC4289
• TNFAIP3 interacting protein 2
• TNFAIP3-interacting protein 2

Background

ABIN-2, TNFAIP3 interacting protein 2, binds to the COOH-terminal zinc finger domain of A20. NF-kB activation induced by TNF and interleukin-1 is inhibited by overexpression of ABIN-2. The latter also inhibits NF-kB activation induced by overexpression of receptor-interacting protein or TNF receptor-associated factor 2. A-20 binding Inhibitor of Nuclear Factor-kappaB (NF-kB)Activation (ABIN-2) binds to A-20. ABIN-2 interferes with NF-kB activation upstream of the IKK complex and might contribute to NF-kB inhibitory function of A-20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNIP2