TMEFF2/Tomoregulin-2 Antibody (J4B6)

Western Blot: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - The Cell and Tissue lysates (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse brain tissue lysate Lane 2.: HeLa cell lysate

Immunocytochemistry/Immunofluorescence: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - ICC/IF analysis of TMEFF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TMEFF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - Human TMEFF2 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Western Blot: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - The Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: SW480 cell lysate

Product Discontinued

TMEFF2/Tomoregulin-2 Antibody (J4B6) Summary

• Immunogen: Recombinant human TMEFF2 (41-150aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMEFF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 41.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TMEFF2/Tomoregulin-2 Antibody (J4B6)

• CT120.2
• HPP1member 2
• Hyperplastic polyposis protein 1
• TENB2
• TMEFF2
• Tomoregulin2
• Tomoregulin-2
• TPEFtomoregulin
• TR-2
• transmembrane protein TENB2
• transmembrane protein with EGF-like and two follistatin-like domains 2
• Transmembrane protein with EGF-like and two follistatin-like domains
• TRtomoregulin-2

Background

TMEFF2(transmembrane protein with EGF-like and two follistatin-like domains 2), a gene encoding a plasma membrane protein with two follistatin-like domains and one epidermal growth factor-like domain, had limited normal tissue distribution and was highly overexpressed in prostate cancer. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. Clone J4B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TMEFF2 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEFF2
• Entrez:
  • 23671(Human)
• Uniprot:
  • Q9UIK5()