TIP60 Antibody - BSA Free

TIP60 antibody detects TIP60 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.TIP60 stained by TIP60 antibody (NBP2-20647) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Jurkat whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TIP60 antibody (NBP2-20647) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TIP60(NBP2-20647) antibody at 1:500 dilution. br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TIP60 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TIP60 Antibody - BSA Free (NBP2-20647) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-TIP60 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TIP60. The exact sequence is proprietary.
• Localization: Nucleus; nucleolus; Cytoplasm; perinuclear region
• Predicted Species: Porcine (99%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KAT5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Reported in scientific literature (PMID: 29320743).
  • Western Blot 1:500-1:3000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TIP60 Antibody - BSA Free

• cPLA2
• ESA1EC 2.3.1.48
• Histone acetyltransferase HTATIP
• histone acetyltransferase KAT5
• HIV-1 Tat interactive protein
• HIV-1 Tat interactive protein, 60kDa
• HTATIP
• HTATIP1
• HTATIPcPLA2 interacting protein
• K(lysine) acetyltransferase 5,60 kDa Tat-interactive protein
• K-acetyltransferase 5
• KAT5
• Lysine acetyltransferase 5
• PLIPTIP
• Tat interacting protein, 60kDa
• TIP60
• TIP60cPLA(2)-interacting protein

Background

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TIP60 Antibody (NBP2-20647)(2)

Publications using NBP2-20647

• Can Liu, Zhongxuan Gui, Cheng An, Fei Sun, Xiaotian Gao, Shenglin Ge STUB1 is acetylated by KAT5 and alleviates myocardial ischemia-reperfusion injury through LATS2-YAP-beta -catenin axis Communications Biology 2024-04-01 [PMID: 38561411]
• Zaini MA, Muller C, de Jong TV et al. A p300 and SIRT1 Regulated Acetylation Switch of C/EBPa Controls Mitochondrial Function Cell Rep 2018-01-09 [PMID: 29320743] (IP, WB, Human)

Bioinformatics

• Gene Symbol: KAT5
• Uniprot:
  • Q92993(Human)