Rat Thyroglobulin ELISA Kit (Colorimetric)

ELISA: Rat Thyroglobulin ELISA Kit (Colorimetric) [NBP3-08184] - Samples were spiked with high concentrations of Rat Thyroglobulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Thyroglobulin ELISA Kit (Colorimetric) [NBP3-08184] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 7.81 - 500 pg/mL
• Sensitivity: 4.69 pg/mL

Rat Thyroglobulin ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 7.81 - 500 pg/mL
• Sensitivity: 4.69 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 6.10
• Intra-Assay: %CV < 6.08
• Spike Recovery: 94 - 110%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Thyroglobulin ELISA Kit (Colorimetric)

• AITD3
• AITD3TGN
• cog
• TDH3
• Tg
• TGN
• Thyroglobulin

Background

Thyroglobulin is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin is the glycoprotein precursor to the thyroid hormones. Its synthesis and metabolism have seemingly wasteful features. It has a molecular weight of 660,000, with 2 identical subunits of MW 300,000 and 10% sugars; yet its complete hydrolysis yields only 2 to 4 molecules of the iodothyroxines T4 and T3. Baas et al. (1986) showed that the TG gene encodes an 8.7-kb mRNA, covers at least 300 kb of genomic DNA and contains at least 37 exons separated by introns as large as 64 kb. A striking structural difference between the 5-prime and 3-prime parts of the gene suggested that it is composed of 2 evolutionarily different regions. The first 30 kb of DNA encodes 3 kb of the mRNA yielding an exon:intron ratio of 1:10, whereas the remaining 270 kb encodes 5.7 kb of the mRNA with a ratio of 1.47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TG