Thyroglobulin Antibody (CL0164) - BSA Free

Western Blot: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Lane 1: Marker [kDa], Lane 2: Human normal thyroid

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Staining of human small intestine shows no positivity.

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Staining of human thyroid gland shows strong cytoplasmic positivity.

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Staining of human thyroid cancer shows strong cytoplasmic positivity.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0164
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Thyroglobulin Antibody (CL0164) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P01266, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Thyroglobulin Recombinant Protein Antigen (NBP2-14784PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Thyroglobulin Antibody (CL0164) - BSA Free

• AITD3
• AITD3TGN
• cog
• TDH3
• Tg
• TGN
• Thyroglobulin

Background

Thyroglobulin is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin is the glycoprotein precursor to the thyroid hormones. Its synthesis and metabolism have seemingly wasteful features. It has a molecular weight of 660,000, with 2 identical subunits of MW 300,000 and 10% sugars; yet its complete hydrolysis yields only 2 to 4 molecules of the iodothyroxines T4 and T3. Baas et al. (1986) showed that the TG gene encodes an 8.7-kb mRNA, covers at least 300 kb of genomic DNA and contains at least 37 exons separated by introns as large as 64 kb. A striking structural difference between the 5-prime and 3-prime parts of the gene suggested that it is composed of 2 evolutionarily different regions. The first 30 kb of DNA encodes 3 kb of the mRNA yielding an exon:intron ratio of 1:10, whereas the remaining 270 kb encodes 5.7 kb of the mRNA with a ratio of 1.47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TG