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THSD4 Antibody

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Western Blot: THSD4 Antibody [NBP2-94253] - Analysis of extracts of SH-SY5Y cells, using THSD4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Western Blot: THSD4 Antibody [NBP2-94253] - Analysis of extracts of SH-SY5Y cells, using THSD4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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THSD4 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human THSD4 (NP_079093.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
THSD4
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for THSD4 Antibody

  • ADAMTSL6
  • EC 3.4.24.14
  • EC 4.2.1.2
  • FLJ13710
  • FVSY9334
  • PRO34005
  • thrombospondin type-1 domain-containing protein 4
  • thrombospondin, type I, domain containing 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Tensin 1 Antibody
NBP1-84129
Immunocytochemistry/Immunofluorescence: Tensin 1 Antibody [NBP1-84129] - Adhesions are more mature and stable on PT compared to SLA. Co-localizations of tensin-1 and p-cortactin with vinculin were shown by immunocytochemical staining of HGFs cultured on PT and SLA surfaces at 24 hrs. Cells were labelled for integrin tensin-1 (red), vinculin (green), and nuclei (blue). Representative images of HGFs on PT and SLA observed under fluorescence microscopy. Grey scale images are shown for separate channels for tensin-1, and vinculin. Inserts show higher magnifications of focal contacts (white boxes). Image collected and cropped by CiteAb from the following publication (http://doi.wiley.com/10.1111/jcmm.12451), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of rat kidneys shows positivity in mesangial cells. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Tensin 1 Antibody [NBP1-84129] - Staining of human liver shows moderate membranous positivity in hepatocytes.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
5-HT4 Antibody
NBP1-78403
Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Species: Hu
Applications: WB, ICC/IF
GSTCD Antibody
H00079807-B01P
Western Blot: GSTCD Antibody [H00079807-B01P] - Analysis of GSTCD expression in A-431.
Western Blot: GSTCD Antibody [H00079807-B01P] - Analysis of GSTCD expression in transfected 293T cell line by GSTCD polyclonal antibody. Lane 1: FLJ13273 transfected lysate(69.52 KDa). Lane 2: Non-transfected lysate.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P
Fibrillin 1
Antibody (11C1.3)
NB110-8146
Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Frozen: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Adult eye (blue) and muscle (red) tissue. An image was captured using epifluorescent microscope detecting signals from Alexa fluor 568 conjugated secondary antibody. Used at 1:100 dilution. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Fibrillin 1 Antibody (11C1.3) [NB110-8146] - Analysis of Fibrillin-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left).
(1 Review)
(3 Publications)
Species: Hu, Po, Bv, Fe
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IM
Hip Antibody
[Unconjugated]
AF1568
Hip Antibody [Unconjugated]
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Species: Mu
Applications: WB
FAM13A Antibody
NBP1-88825
Immunocytochemistry/Immunofluorescence: FAM13A Antibody [NBP1-88825] - Staining of human cell line U-2 OS shows localization to nucleoli, cytosol & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FAM13A Antibody [NBP1-88825] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: FAM13A Antibody [NBP1-88825] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Hexosaminidase
A/HEXA Antibody (7147
MAB6237
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hexosaminidase A/HEXA Monoclonal Antibody (Catalog # MAB6237) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hexosaminidase A/HEXA at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hexosaminidase A/HEXA was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human Hexosaminidase A/HEXA Monoclonal Antibody (Catalog # MAB6237) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and lysosomes in neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: WB, IHC
DPP8 Antibody
(OTI1D2)
NBP2-01830
Western Blot: DPP8 Antibody (OTI1D2) [NBP2-01830] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DPP8 monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: DPP8 Antibody (OTI1D2) [NBP2-01830] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP8 mouse monoclonal antibody.
Western Blot: DPP8 Antibody (OTI1D2) [NBP2-01830] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP8.
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Applications: WB, IHC, IHC-P
ACAT Antibody
NB400-141
ACAT Antibody
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Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IP
Aromatase Antibody
NB100-1596
Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
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Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
PHF22 Antibody
NBP1-92263
Western Blot: PHF22 Antibody [NBP1-92263] - Analysis in human cell line MOLT-4.
Immunocytochemistry/Immunofluorescence: PHF22 Antibody [NBP1-92263] - Staining of human cell line A-431 shows localization to nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PHF22 Antibody [NBP1-92263] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
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Nicotinic
Acetylcholine R alpha ...
NBP2-57262
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody [NBP2-57262] - Analysis in human skeletal muscle and cerebral cortex tissues. Corresponding CHRNA1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody [NBP2-57262] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody [NBP2-57262] - Staining of human liver shows no positivity in hepatocytes as expected.
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Applications: ICC/IF, IHC, IHC-P
NEIL1 Antibody
NBP2-47358
Immunocytochemistry/Immunofluorescence: NEIL1 Antibody [NBP2-47358] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry: NEIL1 Antibody [NBP2-47358] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human salivary gland shows moderate nuclear positivity in glandular cells.
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Bestrophin 1
Antibody (E6-6)
NB300-164
Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) [NB300-164] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm.
Immunohistochemistry-Paraffin: Bestrophin 1 Antibody (E6-6) [NB300-164] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and extracellular.
Western Blot: Bestrophin 1 Antibody (E6-6) [NB300-164] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY licence.
(1 Review)
(41 Publications)
Species: Hu, Mu, Po, Ca, Pm, Rt(-)
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, Dual ISH-IHC
ACAT1 Antibody
NBP1-89284
Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).
Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.
(1 Review)
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Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P, KD
THSD4 Antibody
NBP2-94253
THSD4 Antibody
Species: Hu, Mu
Applications: WB

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Bioinformatics

Gene Symbol THSD4